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KMID : 1098420130210050361
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Korean Journal of Medicinal Crop Science
2013 Volume.21 No. 5 p.361 ~ p.371
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Antimelanogenic Effect and Whitening of Anthocyanin Rich Fraction from Seeds of Liriope platyphylla
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Choung Myoung-Gun
Hwang Young-Sun
Kim Gi-Ppeum
Ah Kyung-Geun
Shim Hoon-Seob
Hong Seung-Beom
Choi Jae-Hoo
Yu Chang-Yeon
Chung Ill-Min
Kim Seung-Hyun
Lim Jung-Dae
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Abstract
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This study was performed to determine the antimelanogenic effect and tyrosinase inhibitory activities of anthocyanin rich fraction (AN-SLP) from Liriope platyphylla Wang et Tang seeds. Anthocyanins isolated from L. platyphylla seeds revealed the presence of four major anthocyanin components, which were tentatively identified as delphinidin-3-Oglucoside, delphinidin-3-O-rutinoside, petunidin-3-O-rutinoside, and malvidin-3-O-rutinoside using semipreparative HPLC, 1 H-NMR, 13C NMR, FAB-MS and LC/ES-MS. The inhibitory effect of AN-SLP on tyrosinase activity was studied using in vitro (against mushroom tyrosinase) and ex vivo (against B16 melanoma cell tyrosinase) models. Cellular tyrosinase activity was decreased by AN-SLP treatment in B 16 melanoma cells through dose dependent manner, but AN-SLP did not inhibit mushroom tyrosinase and L-DOPA oxidation directly. AN-SLP showed melanin inhibition by 53.2% at 50 ㎍/㎖ which was 0.7 times more efficient than the antimelanogenic effect of commercial arbutin and kojic acid (36.5%) also did not show cell toxicity. Additionally, AN-SLP inhibited the activity of α-glucosidase and the glycosylation of tyrosinase in melanoma cell. The resulting unsaturated glycosylation of tyrosinase makes it unstable and disturb correct transportation. From theses results, we conclude that AN-SLP could be used as anti-melanogenic agent for skin whitening.
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KEYWORD
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Anthocyanin Rich Fraction, Antimelanogenic Effect, Seeds of Liriope platyphylla, Tyrosinase Inhibitory Activity
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